Coding

Part:BBa_K2195000:Design

Designed by: Alex Yenkin   Group: iGEM17_WashU_StLouis   (2017-06-16)


Dsup (Radiation Protection)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 240
    Illegal BamHI site found at 694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As this protein came from a eukaryotic species, it was not codon optimized for E. Coli. In addition, there were several illegal restriction sites that had to be replaced with different codons.


Source

This comes from the genomic sequence of the Tardigrade Species Ramazzottius varieornatus. The gene was discovered by Hashimoto et al. (1)

References

1. Hashimoto, T. et al. Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature Communications 7, 12808, doi: 10.1038/ncomms12808 (2016).